2iff: Difference between revisions

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[[Image:2iff.~~gif~~|left|200px]]

[[Image:2iff.png|left|200px]]

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~~'''~~STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION~~'''~~

===STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION===

~~==Overview==~~

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The ~~structure of the complex between the Fab HyHEL-5 and chicken lysozyme revealed a large interface region containing 23 lysozyme and 28 Fab residues. Arg68 of~~ the lysozyme is centrally placed in this interface and theoretical studies together with binding assays of this Fab to different avian lysozymes have previously shown that this arginine residue is an important contributor to the ~~binding. The Arg68-->Lys mutant binds 10~~(~~3) times less well to the HyHEL-5 Fab~~. ~~We have examined the refined crystal structure of the complex of this mutant lysozyme with the Fab~~. ~~No global changes occur~~, ~~but there~~ is ~~an introduction of a new water molecule into the interface that mediates the hydrogen bonding interactions between the lysine and residues on~~ the ~~Fab. These data are compared with the effects of similar changes on the inhibition of serine proteases such as trypsin where the energetic effects of this substitution are small~~.

The line below this paragraph, {{ABSTRACT_PUBMED_7531245}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 7531245 is the PubMed ID number.

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==About this Structure==

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[[Category: Chacko, S.]]

[[Category: Davies, D R.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 07~~:26:46 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:05:21 2008''

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-[[Image:2iff.gif|left|200px]]
+{{Seed}}
+[[Image:2iff.png|left|200px]]
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 {{STRUCTURE_2iff|  PDB=2iff  |  SCENE=  }}
-'''STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION'''
+===STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION===
-==Overview==
+<!--
-The structure of the complex between the Fab HyHEL-5 and chicken lysozyme revealed a large interface region containing 23 lysozyme and 28 Fab residues. Arg68 of the lysozyme is centrally placed in this interface and theoretical studies together with binding assays of this Fab to different avian lysozymes have previously shown that this arginine residue is an important contributor to the binding. The Arg68--&gt;Lys mutant binds 10(3) times less well to the HyHEL-5 Fab. We have examined the refined crystal structure of the complex of this mutant lysozyme with the Fab. No global changes occur, but there is an introduction of a new water molecule into the interface that mediates the hydrogen bonding interactions between the lysine and residues on the Fab. These data are compared with the effects of similar changes on the inhibition of serine proteases such as trypsin where the energetic effects of this substitution are small.
+The line below this paragraph, {{ABSTRACT_PUBMED_7531245}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 7531245 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_7531245}}
 ==About this Structure==
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 [[Category: Chacko, S.]]
 [[Category: Davies, D R.]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:26:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:05:21 2008''