2i94: Difference between revisions

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[[Image:2i94.gif|left|200px]]
{{Seed}}
[[Image:2i94.png|left|200px]]


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{{STRUCTURE_2i94|  PDB=2i94  |  SCENE=  }}  
{{STRUCTURE_2i94|  PDB=2i94  |  SCENE=  }}  


'''NMR Structure of recoverin bound to rhodopsin kinase'''
===NMR Structure of recoverin bound to rhodopsin kinase===




==Overview==
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Recoverin, a member of the neuronal calcium sensor branch of the EF-hand superfamily, serves as a calcium sensor that regulates rhodopsin kinase (RK) activity in retinal rod cells. We report here the NMR structure of Ca(2+)-bound recoverin bound to a functional N-terminal fragment of rhodopsin kinase (residues 1-25, called RK25). The overall main-chain structure of recoverin in the complex is similar to structures of Ca(2+)-bound recoverin in the absence of target (&lt;1.8A root-mean-square deviation). The first eight residues of recoverin at the N terminus are solvent-exposed, enabling the N-terminal myristoyl group to interact with target membranes, and Ca(2+) is bound at the second and third EF-hands of the protein. RK25 in the complex forms an amphipathic helix (residues 4-16). The hydrophobic face of the RK25 helix (Val-9, Val-10, Ala-11, Ala-14, and Phe-15) interacts with an exposed hydrophobic groove on the surface of recoverin lined by side-chain atoms of Trp-31, Phe-35, Phe-49, Ile-52, Tyr-53, Phe-56, Phe-57, Tyr-86, and Leu-90. Residues of recoverin that contact RK25 are highly conserved, suggesting a similar target binding site structure in all neuronal calcium sensor proteins. Site-specific mutagenesis and deletion analysis confirm that the hydrophobic residues at the interface are necessary and sufficient for binding. The recoverin-RK25 complex exhibits Ca(2+)-induced binding to rhodopsin immobilized on concanavalin-A resin. We propose that Ca(2+)-bound recoverin is bound between rhodopsin and RK in a ternary complex on rod outer segment disk membranes, thereby blocking RK interaction with rhodopsin at high Ca(2+).
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{{ABSTRACT_PUBMED_17020884}}


==About this Structure==
==About this Structure==
2I94 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I94 OCA].  
2I94 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I94 OCA].  


==Reference==
==Reference==
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[[Category: Phototransduction and rhodopsin kinse]]
[[Category: Phototransduction and rhodopsin kinse]]
[[Category: Recoverin]]
[[Category: Recoverin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:32:25 2008''

Revision as of 05:32, 28 July 2008

File:2i94.png

Template:STRUCTURE 2i94

NMR Structure of recoverin bound to rhodopsin kinase

Template:ABSTRACT PUBMED 17020884

About this Structure

2I94 is a Protein complex structure of sequences from Bos taurus. Full experimental information is available from OCA.

Reference

Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin., Ames JB, Levay K, Wingard JN, Lusin JD, Slepak VZ, J Biol Chem. 2006 Dec 1;281(48):37237-45. Epub 2006 Oct 4. PMID:17020884

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