2c4b: Difference between revisions

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[[Image:2c4b.~~gif~~|left|200px]]

[[Image:2c4b.png|left|200px]]

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~~'''~~INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A~~'''~~

===INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A===

~~==Overview==~~

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~~We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI~~, ~~a hybrid inhibitor cystine knot microprotein~~, ~~was produced as a soluble fusion~~ to ~~a catalytically inactive variant of~~ the ~~RNAse barnase in Escherichia coli. Functioning~~ as ~~a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination~~. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. ~~Nevertheless~~, ~~the linker region~~ is ~~well ordered in both molecules. This system may prove more generally useful to determine~~ the ~~crystal structure of peptides and small proteins~~.

The line below this paragraph, {{ABSTRACT_PUBMED_16337652}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16337652 is the PubMed ID number.

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==About this Structure==

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[[Category: Serine protease inhibitor]]

[[Category: Squash inhibitor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 21~~:13~~:48~~ 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:36:13 2008''

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-[[Image:2c4b.gif|left|200px]]
+{{Seed}}
+[[Image:2c4b.png|left|200px]]
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 {{STRUCTURE_2c4b|  PDB=2c4b  |  SCENE=  }}
-'''INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A'''
+===INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A===
-==Overview==
+<!--
-We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.
+The line below this paragraph, {{ABSTRACT_PUBMED_16337652}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16337652 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16337652}}
 ==About this Structure==
@@ Line 36: / Line 40: @@
 [[Category: Serine protease inhibitor]]
 [[Category: Squash inhibitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:13:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:36:13 2008''