1zca: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
[[Image:1zca.gif|left|200px]]
{{Seed}}
[[Image:1zca.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1zca|  PDB=1zca  |  SCENE=  }}  
{{STRUCTURE_1zca|  PDB=1zca  |  SCENE=  }}  


'''Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-'''
===Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-===




==Overview==
<!--
The oncogenic G(12/13) subfamily of heterotrimeric G proteins transduces extracellular signals that regulate the actin cytoskeleton, cell cycle progression, and gene transcription. Previously, structural analyses of fully functional G alpha(12/13) subunits have been hindered by insufficient amounts of homogeneous, functional protein. Herein, we report that substitution of the N-terminal helix of G alpha(i1) for the corresponding region of G alpha12 or G alpha13 generated soluble chimeric subunits (G alpha(i/12) and G alpha(i/13)) that could be purified in sufficient amounts for crystallographic studies. Each chimera bound guanine nucleotides, G betagamma subunits, and effector proteins and exhibited GAP responses to p115RhoGEF and leukemia-associated RhoGEF. Like their wild-type counterparts, G alpha(i/13), but not G alpha(i/12), stimulated the activity of p115RhoGEF. Crystal structures of the G alpha(i/12) x GDP x AlF4(-) and G alpha(i/13) x GDP complexes were determined using diffraction data extending to 2.9 and 2.0 A, respectively. These structures reveal not only the native structural features of G alpha12 and G alpha13 subunits, which are expected to be important for their interactions with GPCRs and effectors such as G alpha-regulated RhoGEFs, but also novel conformational changes that are likely coupled to GTP hydrolysis in the G alpha(12/13) class of heterotrimeric G proteins.
The line below this paragraph, {{ABSTRACT_PUBMED_16388592}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16388592 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16388592}}


==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Palmitate]]
[[Category: Palmitate]]
[[Category: Transducer]]
[[Category: Transducer]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:27:09 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:56:41 2008''

Revision as of 05:56, 28 July 2008

File:1zca.png

Template:STRUCTURE 1zca

Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-

Template:ABSTRACT PUBMED 16388592

About this Structure

1ZCA is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

A new approach to producing functional G alpha subunits yields the activated and deactivated structures of G alpha(12/13) proteins., Kreutz B, Yau DM, Nance MR, Tanabe S, Tesmer JJ, Kozasa T, Biochemistry. 2006 Jan 10;45(1):167-74. PMID:16388592

Page seeded by OCA on Mon Jul 28 08:56:41 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1zca&oldid=690919"