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| [[Image:2b5p.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2b5p| PDB=2b5p | SCENE= }} | | {{STRUCTURE_2b5p| PDB=2b5p | SCENE= }} |
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| '''Solution structure of ribbon isoform of CMrVIA lambda conotoxin'''
| | ===Solution structure of ribbon isoform of CMrVIA lambda conotoxin=== |
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| ==Overview==
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| alpha-Conotoxins possess a conserved four-cysteine framework and disulfide linkages (C(1)(-)(3), C(2)(-)(4)) that fold toward the globular conformation with absolute fidelity. Despite the presence of a similar conserved set of cysteine framework, chi/lambda-conotoxins adopt an alternate disulfide-pairing (C(1)(-)(4), C(2)(-)(3)) and its consequent ribbon conformation, exhibiting distinct biological activities from alpha-conotoxins. chi/lambda-Conotoxin CMrVIA (VCCGYKLCHOC-COOH) isolated from the venom of Conus marmoreus natively exists in the ribbon conformation and induces seizures in mice at a potency that is of three orders higher than the non-native globular form. We have chemically synthesized two isoforms of CMrVIA conotoxin in the ribbon and globular conformation and determined their structures by (1)H NMR spectroscopy. The ribbon (PDB ID 2B5P) and globular conformations (PBD ID 2B5Q) were calculated to have paired-wise backbone RMSDs of 0.48 +/- 0.1 and 0.58 +/- 0.1 A respectively. Unlike the native globular alpha-conotoxins, the globular canonical form of CMrVIA chi/lambda-conotoxin exhibited heterogeneity in its solution structure as noted by the presence of minor conformers and poorer RMSD of structure calculation. Paired-wise backbone comparison between the native ribbon and the non-native globular form of CMrVIA conotoxin revealed an RMSD of 4.73 A, emphasizing their distinct conformational differences. These structural data are essential for the understanding of the structure-function activity of chi/lambda-conotoxins, as well as unraveling the folding propensities of these short peptide toxins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16903680}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16903680 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16903680}} |
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| ==About this Structure== | | ==About this Structure== |
| 2B5P is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5P OCA]. | | 2B5P is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5P OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Disulfide linkage]] | | [[Category: Disulfide linkage]] |
| [[Category: Ribbon conformation]] | | [[Category: Ribbon conformation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:53:25 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:11:07 2008'' |
