2bj8: Difference between revisions

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[[Image:2bj8.~~gif~~|left|200px]]

[[Image:2bj8.png|left|200px]]

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~~'''~~NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES~~'''~~

===NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES===

~~==Overview==~~

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The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR) encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity with Escherichia coli nickel responsive repressor NikR (EcNikR), ~~including many residues that are functionally important in~~ the E.~~coli ortholog~~. ~~We succeeded in crystallization and structural characterization of PhNikR in the apo form and two nickel bound forms that exhibit different conformations, open and closed. Moreover~~, ~~we have identified a putative "low-affinity" nickel-binding pocket in~~ the ~~closed form~~. ~~This binding site has unusual nickel coordination and exhibits high sensitivity to phosphate in the crystal structure. Analysis of the PhNikR structures and structure~~-~~based mutational studies with EcNikR reveals a plausible mechanism of nickel~~-~~dependent promoter recognition by the NikR family of proteins.~~

The line below this paragraph, {{ABSTRACT_PUBMED_15826657}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15826657 is the PubMed ID number.

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==About this Structure==

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[[Category: Transcription]]

[[Category: Transcription regulation]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 20~~:21:52 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:27:27 2008''

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-[[Image:2bj8.gif|left|200px]]
+{{Seed}}
+[[Image:2bj8.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2bj8|  PDB=2bj8  |  SCENE=  }}
-'''NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES'''
+===NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES===
-==Overview==
+<!--
-The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR) encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity with Escherichia coli nickel responsive repressor NikR (EcNikR), including many residues that are functionally important in the E.coli ortholog. We succeeded in crystallization and structural characterization of PhNikR in the apo form and two nickel bound forms that exhibit different conformations, open and closed. Moreover, we have identified a putative "low-affinity" nickel-binding pocket in the closed form. This binding site has unusual nickel coordination and exhibits high sensitivity to phosphate in the crystal structure. Analysis of the PhNikR structures and structure-based mutational studies with EcNikR reveals a plausible mechanism of nickel-dependent promoter recognition by the NikR family of proteins.
+The line below this paragraph, {{ABSTRACT_PUBMED_15826657}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15826657 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15826657}}
 ==About this Structure==
@@ Line 35: / Line 39: @@
 [[Category: Transcription]]
 [[Category: Transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:21:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:27:27 2008''