2iie: Difference between revisions

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[[Image:2iie.gif|left|200px]]
{{Seed}}
[[Image:2iie.png|left|200px]]


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{{STRUCTURE_2iie|  PDB=2iie  |  SCENE=  }}  
{{STRUCTURE_2iie|  PDB=2iie  |  SCENE=  }}  


'''single chain Integration Host Factor protein (scIHF2) in complex with DNA'''
===single chain Integration Host Factor protein (scIHF2) in complex with DNA===




==Overview==
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Architectural proteins that reconfigure the paths of DNA segments are required for the establishment of functional interfaces in many genomic transactions. A single-chain derivative of the DNA architectural protein integration host factor was found to adopt two stable conformational states in complex with a specific DNA target. In the so-called open state, the degree of protein-induced DNA bending is reduced significantly compared with the closed state. The conformational switch between these states is controlled by divalent metal binding in two electronegative zones arising from the lysine-to-glutamate substitution in the protein body proximal to the phosphate backbone of one DNA arm. We show that this switch can be employed to control the efficiency of site-specific recombination catalyzed by lambda integrase. Introduction of acidic residues at the protein-DNA interface holds potential for the design of metal-mediated switches for the investigation of functional relationships.
The line below this paragraph, {{ABSTRACT_PUBMED_17276457}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17276457 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17276457}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A divalent metal-mediated switch controlling protein-induced DNA bending., Bao Q, Chen H, Liu Y, Yan J, Droge P, Davey CA, J Mol Biol. 2007 Mar 30;367(3):731-40. Epub 2006 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17276457 17276457]
A divalent metal-mediated switch controlling protein-induced DNA bending., Bao Q, Chen H, Liu Y, Yan J, Droge P, Davey CA, J Mol Biol. 2007 Mar 30;367(3):731-40. Epub 2006 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17276457 17276457]
Activation of site-specific DNA integration in human cells by a single chain integration host factor., Corona T, Bao Q, Christ N, Schwartz T, Li J, Droge P, Nucleic Acids Res. 2003 Sep 1;31(17):5140-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12930965 12930965]
Single-chain integration host factors as probes for high-precision nucleoprotein complex formation., Bao Q, Christ N, Droge P, Gene. 2004 Dec 8;343(1):99-106. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15563835 15563835]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Metal]]
[[Category: Metal]]
[[Category: U-turn]]
[[Category: U-turn]]
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