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| {{STRUCTURE_2nr9| PDB=2nr9 | SCENE= }} | | {{STRUCTURE_2nr9| PDB=2nr9 | SCENE= }} |
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| '''Crystal structure of GlpG, Rhomboid Peptidase from Haemophilus influenzae'''
| | ===Crystal structure of GlpG, Rhomboid Peptidase from Haemophilus influenzae=== |
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| ==Overview==
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| Rhomboid peptidases are members of a family of regulated intramembrane peptidases that cleave the transmembrane segments of integral membrane proteins. Rhomboid peptidases have been shown to play a major role in developmental processes in Drosophila and in mitochondrial maintenance in yeast. Most recently, the function of rhomboid peptidases has been directly linked to apoptosis. We have solved the structure of the rhomboid peptidase from Haemophilus influenzae (hiGlpG) to 2.2-A resolution. The phasing for the crystals of hiGlpG was provided mainly by molecular replacement, by using the coordinates of the Escherichia coli rhomboid (ecGlpG). The structural results on these rhomboid peptidases have allowed us to speculate on the catalytic mechanism of substrate cleavage in a membranous environment. We have identified the relative disposition of the nucleophilic serine to the general base/acid function of the conserved histidine. Modeling a tetrapeptide substrate in the context of the rhomboid structure reveals an oxyanion hole comprising the side chain of a second conserved histidine and the main-chain NH of the nucleophilic serine residue. In both hiGlpG and ecGlpG structures, a water molecule occupies this oxyanion hole.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17210913}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17210913 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17210913}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rhomboid protease]] | | [[Category: Rhomboid protease]] |
| [[Category: X-ray crystallography]] | | [[Category: X-ray crystallography]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:48:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:57:19 2008'' |
