2hgl: Difference between revisions

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[[Image:2hgl.gif|left|200px]]
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[[Image:2hgl.png|left|200px]]


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{{STRUCTURE_2hgl|  PDB=2hgl  |  SCENE=  }}  
{{STRUCTURE_2hgl|  PDB=2hgl  |  SCENE=  }}  


'''NMR structure of the first qRRM domain of human hnRNP F'''
===NMR structure of the first qRRM domain of human hnRNP F===




==Overview==
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The heterogeneous nuclear ribonucleoprotein (hnRNP) F belongs to the hnRNP H family involved in the regulation of alternative splicing and polyadenylation and specifically recognizes poly(G) sequences (G-tracts). In particular, hnRNP F binds a G-tract of the Bcl-x RNA and regulates its alternative splicing, leading to two isoforms, Bcl-x(S) and Bcl-x(L), with antagonist functions. In order to gain insight into G-tract recognition by hnRNP H members, we initiated an NMR study of human hnRNP F. We present the solution structure of the three quasi RNA recognition motifs (qRRMs) of hnRNP F and identify the residues that are important for the interaction with the Bcl-x RNA by NMR chemical shift perturbation and mutagenesis experiments. The three qRRMs exhibit the canonical betaalphabetabetaalphabeta RRM fold but additional secondary structure elements are present in the two N-terminal qRRMs of hnRNP F. We show that qRRM1 and qRRM2 but not qRRM3 are responsible for G-tract recognition and that the residues of qRRM1 and qRRM2 involved in G-tract interaction are not on the beta-sheet surface as observed for the classical RRM but are part of a short beta-hairpin and two adjacent loops. These regions define a novel interaction surface for RNA recognition by RRMs.
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{{ABSTRACT_PUBMED_16885237}}


==About this Structure==
==About this Structure==
2HGL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HGL OCA].  
2HGL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HGL OCA].  


==Reference==
==Reference==
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[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
[[Category: Splicing]]
[[Category: Splicing]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:16:10 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:39:41 2008''

Revision as of 08:39, 28 July 2008

File:2hgl.png

Template:STRUCTURE 2hgl

NMR structure of the first qRRM domain of human hnRNP F

Template:ABSTRACT PUBMED 16885237

About this Structure

2HGL is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition., Dominguez C, Allain FH, Nucleic Acids Res. 2006 Aug 2;34(13):3634-45. Print 2006. PMID:16885237

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