|
|
| Line 1: |
Line 1: |
| [[Image:2ihj.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2ihj.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2ihj| PDB=2ihj | SCENE= }} | | {{STRUCTURE_2ihj| PDB=2ihj | SCENE= }} |
|
| |
|
| '''crystal structure of multifunctional sialyltransferase from pasteurella multocida with CMP-3F-Neu5Ac bound'''
| | ===crystal structure of multifunctional sialyltransferase from pasteurella multocida with CMP-3F-Neu5Ac bound=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Sialyltransferases are key enzymes involved in the biosynthesis of biologically and pathologically important sialic acid-containing molecules in nature. Binary X-ray crystal structures of a multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1) with a donor analogue CMP-3F(a)Neu5Ac or CMP-3F(e)Neu5Ac were determined at 2.0 and 1.9 A resolutions, respectively. Ternary X-ray structures of the protein in complex with CMP or a donor analogue CMP-3F(a)Neu5Ac and an acceptor lactose have been determined at 2.0 and 2.27 A resolutions, respectively. This represents the first sialyltransferase structure and the first GT-B-type glycosyltransferase structure that is bound to both a donor analogue and an acceptor simultaneously. The four structures presented here reveal that binding of the nucleotide-activated donor sugar causes a buried tryptophan to flip out of the protein core to interact with the donor sugar and helps define the acceptor sugar binding site. Additionally, key amino acid residues involved in the catalysis have been identified. Structural and kinetic data support a direct displacement mechanism involving an oxocarbenium ion-like transition state assisted with Asp141 serving as a general base to activate the acceptor hydroxyl group.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17487984}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17487984 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_17487984}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 26: |
Line 30: |
| [[Category: Ni, L.]] | | [[Category: Ni, L.]] |
| [[Category: Cmp-3f-neu5ac sialyltransferase two-rossmann domain]] | | [[Category: Cmp-3f-neu5ac sialyltransferase two-rossmann domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:30:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:40:17 2008'' |
