2ffw: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:2ffw.gif|left|200px]]
{{Seed}}
[[Image:2ffw.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ffw|  PDB=2ffw  |  SCENE=  }}  
{{STRUCTURE_2ffw|  PDB=2ffw  |  SCENE=  }}  


'''Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING'''
===Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING===




==Overview==
<!--  
B-box domains are a defining feature of the tripartite RBCC (RING, B-box, coiled-coil) or TRIM proteins, many of which are E3 ubiquitin ligases. However, little is known about the biological function of B-boxes. In some RBCC/TRIM proteins there is only a single B-box (type 2) domain, while others have both type 1 and type 2 B-box domains in tandem adjacent to their RING domain. These two types of B-boxes share little sequence similarity, except the presence of cysteine and histidine residues: eight in most B-box1 domains and seven in B-box2 domains. We report here the high-resolution solution structure of the first B-box1 domain (from the human RBCC protein, MID1) based on 670 nuclear Overhauser effect (NOE)-derived distance restraints, 12 hydrogen bonds, and 44 dihedral angles. The domain consists of a three-turn alpha-helix, two short beta-strands, and three beta-turns, encompassing Val117 to Pro164, which binds two zinc atoms. One zinc atom is coordinated by cysteine residues 119, 122, 142, 145, while cysteine 134, 137 and histidine 150, 159 coordinate the other. This topology is markedly different from the only other B-box structure reported; that of a type 2 B-box from Xenopus XNF7, which binds a single zinc atom. Of note, the B-box1 structure closely resembles the folds of the RING, ZZ and U-box domains of E3 and E4 ubiquitin enzymes, raising the possibility that the B-box1 domain either has E3 activity itself or enhances the activity of RING type E3 ligases (i.e. confers E4 enzyme activity). The structure of the MID1 B-box1 also reveals two potential protein interaction surfaces. One of these is likely to provide the binding interface for Alpha 4 that is required for the localized turnover of the catalytic subunit of PP2A, the major Ser/Thr phosphatase.
The line below this paragraph, {{ABSTRACT_PUBMED_16529770}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16529770 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16529770}}


==About this Structure==
==About this Structure==
2FFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFW OCA].  
2FFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFW OCA].  


==Reference==
==Reference==
Line 30: Line 34:
[[Category: Ring finger]]
[[Category: Ring finger]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:50:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:42:11 2008''

Revision as of 08:42, 28 July 2008

File:2ffw.png

Template:STRUCTURE 2ffw

Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING

Template:ABSTRACT PUBMED 16529770

About this Structure

2FFW is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING., Massiah MA, Simmons BN, Short KM, Cox TC, J Mol Biol. 2006 Apr 28;358(2):532-45. Epub 2006 Feb 20. PMID:16529770

Page seeded by OCA on Mon Jul 28 11:42:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2ffw&oldid=696834"