1ryh: Difference between revisions

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[[Image:1ryh.~~gif~~|left|200px]]

[[Image:1ryh.png|left|200px]]

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~~'''~~Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase~~'''~~

===Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase===

~~==Overview==~~

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~~Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region.~~ The ~~structures of Rac1b in~~ the ~~GDP- and~~ the ~~GppNHp-bound forms, determined~~ at ~~a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II~~. ~~As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins~~. ~~Interestingly~~, ~~Rac1b~~ is ~~able to bind~~ the ~~GTPase~~-~~binding domain of PAK but not full~~-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.

The line below this paragraph, {{ABSTRACT_PUBMED_14625275}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 14625275 is the PubMed ID number.

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==About this Structure==

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[[Category: Fiegen, D.]]

[[Category: Gtp binding]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:03:52 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:00:18 2008''

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-[[Image:1ryh.gif|left|200px]]
+{{Seed}}
+[[Image:1ryh.png|left|200px]]
 <!--
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 {{STRUCTURE_1ryh|  PDB=1ryh  |  SCENE=  }}
-'''Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase'''
+===Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase===
-==Overview==
+<!--
-Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region. The structures of Rac1b in the GDP- and the GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Interestingly, Rac1b is able to bind the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.
+The line below this paragraph, {{ABSTRACT_PUBMED_14625275}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 14625275 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_14625275}}
 ==About this Structure==
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 [[Category: Fiegen, D.]]
 [[Category: Gtp binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:03:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:00:18 2008''