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[[Image:3eng.jpg|left|200px]]
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{{STRUCTURE_3eng|  PDB=3eng  |  SCENE=  }}  
{{STRUCTURE_3eng|  PDB=3eng  |  SCENE=  }}  


'''STRUCTURE OF ENDOGLUCANASE V CELLOBIOSE COMPLEX'''
===STRUCTURE OF ENDOGLUCANASE V CELLOBIOSE COMPLEX===




==Overview==
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The structure of the catalytic core of the endoglucanase V (EGV) from Humicola insolens has been determined by the method of multiple isomorphous replacement at 1.5 A resolution. The final model, refined with X-PLOR and PROLSQ, has a crystallographic R factor of 0.163 (R(free) = 0.240) with deviations from stereochemical target values of 0.012 A and 0.037 degrees for bonds and angles, respectively. The model was further refined with SHELXL, including anisotropic modelling of the protein-atom temperature factors, to give a final model with an R factor of 0.105 and an R(free) of 0.154. The initial isomorphous replacement electron-density map was poor and uninterpretable but was improved by the use of synchrotron data collected at a wavelength chosen so as to optimize the f" contribution of the anomalous scattering from the heavy atoms. The structure of H. insolens EGV consists of a six-stranded beta-barrel domain, similar to that found in a family of plant defence proteins, linked by a number of disulfide-bonded loop regions. A long open groove runs across the surface of the enzyme either side of which lie the catalytic aspartate residues. The 9 A separation of the catalytic carboxylate groups is consistent with the observation that EGV catalyzes the hydrolysis of the cellulose, beta(1--&gt;4) links with inversion of configuration at the anomeric C1 atom. This structure is the first representative from the glycosyl hydrolase family 45.
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{{ABSTRACT_PUBMED_15299721}}


==About this Structure==
==About this Structure==
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[[Category: Glycosyl hydrolase]]
[[Category: Glycosyl hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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Revision as of 09:00, 28 July 2008

File:3eng.png

Template:STRUCTURE 3eng

STRUCTURE OF ENDOGLUCANASE V CELLOBIOSE COMPLEX

Template:ABSTRACT PUBMED 15299721

About this Structure

3ENG is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.

Reference

Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5 A resolution., Davies GJ, Dodson G, Moore MH, Tolley SP, Dauter Z, Wilson KS, Rasmussen G, Schulein M, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):7-17. PMID:15299721

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