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[[Image:2flq.gif|left|200px]]
{{Seed}}
[[Image:2flq.png|left|200px]]


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{{STRUCTURE_2flq|  PDB=2flq  |  SCENE=  }}  
{{STRUCTURE_2flq|  PDB=2flq  |  SCENE=  }}  


'''Crystal Structure of Nitric Oxide Synthase from Geobacillus Stearothermophilus (ATCC 12980) complexed with L-arginine'''
===Crystal Structure of Nitric Oxide Synthase from Geobacillus Stearothermophilus (ATCC 12980) complexed with L-arginine===




==Overview==
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In an effort to generate more stable reaction intermediates involved in substrate oxidation by nitric-oxide synthases (NOSs), we have cloned, expressed, and characterized a thermostable NOS homolog from the thermophilic bacterium Geobacillus stearothermophilus (gsNOS). As expected, gsNOS forms nitric oxide (NO) from l-arginine via the stable intermediate N-hydroxy l-arginine (NOHA). The addition of oxygen to ferrous gsNOS results in long-lived heme-oxy complexes in the presence (Soret peak 427 nm) and absence (Soret peak 413 nm) of substrates l-arginine and NOHA. The substrate-induced red shift correlates with hydrogen bonding between substrate and heme-bound oxygen resulting in conversion to a ferric heme-superoxy species. In single turnover experiments with NOHA, NO forms only in the presence of H(4)B. The crystal structure of gsNOS at 3.2 AA of resolution reveals great similarity to other known bacterial NOS structures, with the exception of differences in the distal heme pocket, close to the oxygen binding site. In particular, a Lys-356 (Bacillus subtilis NOS) to Arg-365 (gsNOS) substitution alters the conformation of a conserved Asp carboxylate, resulting in movement of an Ile residue toward the heme. Thus, a more constrained heme pocket may slow ligand dissociation and increase the lifetime of heme-bound oxygen to seconds at 4 degrees C. Similarly, the ferric-heme NO complex is also stabilized in gsNOS. The slow kinetics of gsNOS offer promise for studying downstream intermediates involved in substrate oxidation.
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==About this Structure==
==About this Structure==
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[[Category: Nitric oxide synthase]]
[[Category: Nitric oxide synthase]]
[[Category: Thermostable enzyme]]
[[Category: Thermostable enzyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:02:36 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:14:57 2008''

Revision as of 09:15, 28 July 2008

File:2flq.png

Template:STRUCTURE 2flq

Crystal Structure of Nitric Oxide Synthase from Geobacillus Stearothermophilus (ATCC 12980) complexed with L-arginine

Template:ABSTRACT PUBMED 16407211

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure and reactivity of a thermostable prokaryotic nitric-oxide synthase that forms a long-lived oxy-heme complex., Sudhamsu J, Crane BR, J Biol Chem. 2006 Apr 7;281(14):9623-32. Epub 2006 Jan 11. PMID:16407211

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