1smf: Difference between revisions

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[[Image:1smf.gif|left|200px]]
{{Seed}}
[[Image:1smf.png|left|200px]]


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{{STRUCTURE_1smf|  PDB=1smf  |  SCENE=  }}  
{{STRUCTURE_1smf|  PDB=1smf  |  SCENE=  }}  


'''STUDIES ON AN ARTIFICIAL TRYPSIN INHIBITOR PEPTIDE DERIVED FROM THE MUNG BEAN INHIBITOR'''
===STUDIES ON AN ARTIFICIAL TRYPSIN INHIBITOR PEPTIDE DERIVED FROM THE MUNG BEAN INHIBITOR===




==Overview==
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The active fragment with Lys at the reactive site of mung bean trypsin inhibitor (MBILF) is composed of two peptide chains, A1 of 26 residues and A2 of 9 residues linked via two disulfide bonds. In the present study, a peptide of 22 residue comprising the sequence of chain A1 from position 3 to 24 was synthesized by the solid-phase method. This synthetic peptide with six Cys residues contains a reactive site at position Lys11I-Ser12I (I denotes an inhibitor residue). Air oxidation and HPLC purification resulted in two antitrypsin active components, SPC1 and SPC2. Neither SPC1 nor SPC2 can stoichiometrically inhibit trypsin. The Ki values of SPC1 and SPC2 are 1.2 x 10(-7) and 4.0 x 10(-8) M, respectively. The complexes of SPC1 and SPC2 with bovine beta-trypsin (BTRY) were crystallized by ammonium sulphate precipitation at pH 6.4 and 6.0, respectively. The two crystals have the same crystal form with space group P2(1)2(1)2(1) and cell dimension of a = 63.2(2) A, b = 63.5(6) A, and c = 69.8(4) A. The crystal structure of one complex, SPC1-BTRY, was determined and refined at 2.2 A resolution to a final R-value of 19.2%. From the resulting electron density map, 9 residues of SPC1, from position 9I to 17I, were identified clearly and three-dimension atomic model of the 9-residue reactive loop formed by a disulfide bridge, Cys9I-Cys17I, was built. No electron density corresponding to the other 13 residues was observed in the present map.(ABSTRACT TRUNCATED AT 250 WORDS)
The line below this paragraph, {{ABSTRACT_PUBMED_7798176}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_7798176}}


==About this Structure==
==About this Structure==
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[[Category: Tang, Y.]]
[[Category: Tang, Y.]]
[[Category: Zhang, S.]]
[[Category: Zhang, S.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:53:09 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:34:05 2008''

Revision as of 09:34, 28 July 2008

File:1smf.png

Template:STRUCTURE 1smf

STUDIES ON AN ARTIFICIAL TRYPSIN INHIBITOR PEPTIDE DERIVED FROM THE MUNG BEAN INHIBITOR

Template:ABSTRACT PUBMED 7798176

About this Structure

1SMF is a Protein complex structure. Full crystallographic information is available from OCA.

Reference

Studies on an artificial trypsin inhibitor peptide derived from the mung bean trypsin inhibitor: chemical synthesis, refolding, and crystallographic analysis of its complex with trypsin., Li Y, Huang Q, Zhang S, Liu S, Chi C, Tang Y, J Biochem. 1994 Jul;116(1):18-25. PMID:7798176

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