1yf6: Difference between revisions

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[[Image:1yf6.gif|left|200px]]
{{Seed}}
[[Image:1yf6.png|left|200px]]


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{{STRUCTURE_1yf6|  PDB=1yf6  |  SCENE=  }}  
{{STRUCTURE_1yf6|  PDB=1yf6  |  SCENE=  }}  


'''Structure of a quintuple mutant of photosynthetic reaction center from rhodobacter sphaeroides'''
===Structure of a quintuple mutant of photosynthetic reaction center from rhodobacter sphaeroides===




==Overview==
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The photosynthetic reaction center (RC) from purple bacteria converts light into chemical energy. Although the RC shows two nearly structurally symmetric branches, A and B, light-induced electron transfer in the native RC occurs almost exclusively along the A-branch to a primary quinone electron acceptor Q(A). Subsequent electron and proton transfer to a mobile quinone molecule Q(B) converts it to a quinol, Q(B)H(2). We report the construction and characterization of a series of mutants in Rhodobacter sphaeroides designed to reduce Q(B) via the B-branch. The quantum efficiency to Q(B) via the B-branch Phi(B) ranged from 0.4% in an RC containing the single mutation Ala-M260 --&gt; Trp to 5% in a quintuple mutant which includes in addition three mutations to inhibit transfer along the A-branch (Gly-M203 --&gt; Asp, Tyr-M210 --&gt; Phe, Leu-M214 --&gt; His) and one to promote transfer along the B-branch (Phe-L181 --&gt; Tyr). Comparing the value of 0.4% for Phi(B) obtained in the AW(M260) mutant, which lacks Q(A), to the 100% quantum efficiency for Phi(A) along the A-branch in the native RC, we obtain a ratio for A-branch to B-branch electron transfer of 250:1. We determined the structure of the most effective (quintuple) mutant RC at 2.25 A (R-factor = 19.6%). The Q(A) site did not contain a quinone but was occupied by the side chain of Trp-M260 and a Cl(-). In this structure a nonfunctional quinone was found to occupy a new site near M258 and M268. The implications of this work to trap intermediate states are discussed.
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{{ABSTRACT_PUBMED_15865437}}


==About this Structure==
==About this Structure==
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[[Category: Quinone movement]]
[[Category: Quinone movement]]
[[Category: Rhodobacter sphaeroide]]
[[Category: Rhodobacter sphaeroide]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:14:44 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:40:16 2008''

Revision as of 09:40, 28 July 2008

File:1yf6.png

Template:STRUCTURE 1yf6

Structure of a quintuple mutant of photosynthetic reaction center from rhodobacter sphaeroides

Template:ABSTRACT PUBMED 15865437

About this Structure

1YF6 is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Quinone (QB) reduction by B-branch electron transfer in mutant bacterial reaction centers from Rhodobacter sphaeroides: quantum efficiency and X-ray structure., Paddock ML, Chang C, Xu Q, Abresch EC, Axelrod HL, Feher G, Okamura MY, Biochemistry. 2005 May 10;44(18):6920-8. PMID:15865437

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