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| [[Image:1tbk.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1tbk| PDB=1tbk | SCENE= }} | | {{STRUCTURE_1tbk| PDB=1tbk | SCENE= }} |
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| '''NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.'''
| | ===NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.=== |
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| ==Overview==
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| An important step in the substrate recognition of the Neurospora Varkud Satellite (VS) ribozyme is the formation of a magnesium-dependent loop/loop interaction between the terminal loops of stem-loops I and V. We have studied the structure of stem-loop V by nuclear magnetic resonance spectroscopy and shown that it adopts a U-turn conformation, a common motif found in RNA. Structural comparisons indicate that the U-turn of stem-loop V fulfills some but not all of the structural characteristics found in canonical U-turn structures. This U-turn conformation exposes the Watson-Crick faces of the bases within stem-loop V (G697, A698, and C699) and makes them accessible for interaction with stem-loop I. Using chemical-shift mapping, we show that magnesium ions interact with the loop of the isolated stem-loop V and induce a conformational change that may be important for interaction with stem-loop I. This study expands our understanding of the role of U-turn motifs in RNA structure and function and provides insights into the mechanism of substrate recognition in the VS ribozyme.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15766243}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15766243 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15766243}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBK OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBK OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Hairpin]] | | [[Category: Hairpin]] |
| [[Category: U-turn]] | | [[Category: U-turn]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:46:10 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:44:43 2008'' |
