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| [[Image:1yod.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1yod| PDB=1yod | SCENE= }} | | {{STRUCTURE_1yod| PDB=1yod | SCENE= }} |
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| '''Crystal structure of a water soluble analog of phospholamban'''
| | ===Crystal structure of a water soluble analog of phospholamban=== |
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| ==Overview==
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| Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15826670}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15826670 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15826670}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein design]] | | [[Category: Protein design]] |
| [[Category: Water-soluble]] | | [[Category: Water-soluble]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:34:43 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:52:13 2008'' |
