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| [[Image:2jxm.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2jxm| PDB=2jxm | SCENE= }} | | {{STRUCTURE_2jxm| PDB=2jxm | SCENE= }} |
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| '''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex'''
| | ===Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex=== |
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| ==Overview==
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| The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state. | | The line below this paragraph, {{ABSTRACT_PUBMED_18201089}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18201089 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18201089}} |
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| ==About this Structure== | | ==About this Structure== |
| 2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. | | 2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Metal-binding]] | | [[Category: Metal-binding]] |
| [[Category: Transport]] | | [[Category: Transport]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:56:34 2008'' |
