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| [[Image:2fl1.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2fl1| PDB=2fl1 | SCENE= }} | | {{STRUCTURE_2fl1| PDB=2fl1 | SCENE= }} |
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| '''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
| | ===Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution=== |
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| ==Overview==
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| The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum. | | The line below this paragraph, {{ABSTRACT_PUBMED_16627946}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16627946 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16627946}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Zoanthus sp.]] | | [[Category: Zoanthus sp.]] |
| [[Category: Zrfp574]] | | [[Category: Zrfp574]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:00:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:04:08 2008'' |
