2b7t: Difference between revisions

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[[Image:2b7t.gif|left|200px]]
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[[Image:2b7t.png|left|200px]]


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{{STRUCTURE_2b7t|  PDB=2b7t  |  SCENE=  }}  
{{STRUCTURE_2b7t|  PDB=2b7t  |  SCENE=  }}  


'''Structure of ADAR2 dsRBM1'''
===Structure of ADAR2 dsRBM1===




==Overview==
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Adenosine deaminases that act on RNA (ADARs) site-selectively modify adenosines to inosines within RNA transcripts, thereby recoding genomic information. How ADARs select specific adenosine moieties for deamination is poorly understood. Here, we report NMR structures of the two double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR chemical shift perturbation study of the interaction of the two dsRBMs with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes two bulged bases adjacent to the editing site, demonstrating RNA structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis studies with both the protein and the RNA further support our structural findings.
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{{ABSTRACT_PUBMED_16472753}}


==About this Structure==
==About this Structure==
2B7T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7T OCA].  
2B7T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7T OCA].  


==Reference==
==Reference==
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[[Category: Rna editing]]
[[Category: Rna editing]]
[[Category: Rna-binding protein]]
[[Category: Rna-binding protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:57:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:05:15 2008''

Revision as of 10:05, 28 July 2008

File:2b7t.png

Template:STRUCTURE 2b7t

Structure of ADAR2 dsRBM1

Template:ABSTRACT PUBMED 16472753

About this Structure

2B7T is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs., Stefl R, Xu M, Skrisovska L, Emeson RB, Allain FH, Structure. 2006 Feb;14(2):345-55. PMID:16472753

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