2qpe: Difference between revisions

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[[Image:2qpe.jpg|left|200px]]
{{Seed}}
[[Image:2qpe.png|left|200px]]


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{{STRUCTURE_2qpe|  PDB=2qpe  |  SCENE=  }}  
{{STRUCTURE_2qpe|  PDB=2qpe  |  SCENE=  }}  


'''An unexpected outcome of surface-engineering an integral membrane protein: Improved crystallization of cytochrome ba3 oxidase from Thermus thermophilus'''
===An unexpected outcome of surface-engineering an integral membrane protein: Improved crystallization of cytochrome ba3 oxidase from Thermus thermophilus===




==Overview==
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Cytochrome ba(3) oxidase is an integral membrane protein identified in the thermophilic bacterium Thermus thermophilus. The enzyme has now been expressed recombinantly and purified with a histidine tag. As such, it crystallizes under similar conditions and in the same space group (P4(3)2(1)2) as the native protein. A novel cryoprotection scheme is described here to obtain high-resolution diffraction from these crystals, which involves soaking in a mixture of glycerol and ethylene glycol under a layer of oil. The unit-cell parameters for these crystals are larger than the native protein, apparently deriving from increased ordering of the N-terminus and an internal loop (residues 495-500) in subunit I. Hence, compared with native cytochrome ba(3) oxidase, the recombinant His-tagged protein is accommodated in an expanded but equally well ordered lattice via an alternate set of specific intermolecular contacts. The structure was refined against data to 2.3 angstroms resolution to an R factor of 21.7% and an R(free) of 23.7%.
The line below this paragraph, {{ABSTRACT_PUBMED_15735345}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15735345 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15735345}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A novel cryoprotection scheme for enhancing the diffraction of crystals of recombinant cytochrome ba3 oxidase from Thermus thermophilus., Hunsicker-Wang LM, Pacoma RL, Chen Y, Fee JA, Stout CD, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):340-3. Epub 2005, Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15735345 15735345]
A novel cryoprotection scheme for enhancing the diffraction of crystals of recombinant cytochrome ba3 oxidase from Thermus thermophilus., Hunsicker-Wang LM, Pacoma RL, Chen Y, Fee JA, Stout CD, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):340-3. Epub 2005, Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15735345 15735345]
A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8., Chen Y, Hunsicker-Wang L, Pacoma RL, Luna E, Fee JA, Protein Expr Purif. 2005 Apr;40(2):299-318. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15766872 15766872]
[[Category: Cytochrome-c oxidase]]
[[Category: Cytochrome-c oxidase]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Transmembrane]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport]]
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