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| {{STRUCTURE_2a5c| PDB=2a5c | SCENE= }} | | {{STRUCTURE_2a5c| PDB=2a5c | SCENE= }} |
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| '''Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine'''
| | ===Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine=== |
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| ==Overview==
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| Oxidative damage of DNA results in the formation of many products, including 8-oxodeoxyguanosine, which has been used as a marker to quantify DNA damage. Earlier studies have demonstrated that avidin, a protein prevalent in egg-white and which has high affinity for the vitamin biotin, binds to 8-oxodeoxyguanosine and related bases. In this study, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeoxyadenosine. In each case, the base is observed to bind within the biotin-binding site of avidin. However, the mode of association between the bases and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein in this region does not accompany binding. Fluorescence studies indicate that in solution the individual bases, and a range of oligonucleotides, bind to avidin with micromolar affinity. Only one of the modes of binding observed is consistent with recognition of oxidised purines when incorporated within a DNA oligomer, and from this structure a model is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanosine. These studies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the low levels of binding observed are inconsistent with the recognition of oxidised purines forming a major physiological role for avidin.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16413579}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16413579 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16413579}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Damaged dna]] | | [[Category: Damaged dna]] |
| [[Category: X-ray crystallography]] | | [[Category: X-ray crystallography]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:30 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:06:24 2008'' |
