1t0o: Difference between revisions

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[[Image:1t0o.gif|left|200px]]
{{Seed}}
[[Image:1t0o.png|left|200px]]


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{{STRUCTURE_1t0o|  PDB=1t0o  |  SCENE=  }}  
{{STRUCTURE_1t0o|  PDB=1t0o  |  SCENE=  }}  


'''The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose'''
===The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose===




==Overview==
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The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.
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{{ABSTRACT_PUBMED_15136043}}


==About this Structure==
==About this Structure==
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[[Category: Beta-d-galactose]]
[[Category: Beta-d-galactose]]
[[Category: Glycoprotein,complex]]
[[Category: Glycoprotein,complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:21:36 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:41:58 2008''

Revision as of 11:42, 28 July 2008

File:1t0o.png

Template:STRUCTURE 1t0o

The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose

Template:ABSTRACT PUBMED 15136043

About this Structure

1T0O is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043

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