2d0a: Difference between revisions

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[[Image:2d0a.gif|left|200px]]
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[[Image:2d0a.png|left|200px]]


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{{STRUCTURE_2d0a|  PDB=2d0a  |  SCENE=  }}  
{{STRUCTURE_2d0a|  PDB=2d0a  |  SCENE=  }}  


'''Crystal structure of Bst-RNase HIII'''
===Crystal structure of Bst-RNase HIII===




==Overview==
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Ribonuclease HIII (Bst-RNase HIII) from the moderate thermophile Bacillus stearothermophilus is a type 2 RNase H but shows poor amino acid sequence identity with another type 2 RNase H, RNase HII. It is composed of 310 amino acid residues and acts as a monomer. Bst-RNase HIII has a large N-terminal extension with unknown function and a unique active-site motif (DEDE), both of which are characteristics common to RNases HIII. To understand the role of these N-terminal extension and active-site residues, the crystal structure of Bst-RNase HIII was determined in both metal-free and metal-bound forms at 2.1-2.6 angstroms resolutions. According to these structures, Bst-RNase HIII consists of the N-terminal domain and C-terminal RNase H domain. The structures of the N and C-terminal domains were similar to those of TATA-box binding proteins and archaeal RNases HII, respectively. The steric configurations of the four conserved active-site residues were very similar to those of other type 1 and type 2 RNases H. Single Mn and Mg ions were coordinated with Asp97, Glu98, and Asp202, which correspond to Asp10, Glu48, and Asp70 of Escherichia coli RNase HI, respectively. The mutational studies indicated that the replacement of either one of these residues with Ala resulted in a great reduction of the enzymatic activity. Overproduction, purification, and characterization of the Bst-RNase HIII derivatives with N and/or C-terminal truncations indicated that the N-terminal domain and C-terminal helix are involved in substrate binding, but the former contributes to substrate binding more greatly than the latter.
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{{ABSTRACT_PUBMED_16343535}}


==About this Structure==
==About this Structure==
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[[Category: Ribonuclease h]]
[[Category: Ribonuclease h]]
[[Category: Rna/dna hybrid]]
[[Category: Rna/dna hybrid]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:27:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:10:31 2008''

Revision as of 12:10, 28 July 2008

File:2d0a.png

Template:STRUCTURE 2d0a

Crystal structure of Bst-RNase HIII

Template:ABSTRACT PUBMED 16343535

About this Structure

2D0A is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure and structure-based mutational analyses of RNase HIII from Bacillus stearothermophilus: a new type 2 RNase H with TBP-like substrate-binding domain at the N terminus., Chon H, Matsumura H, Koga Y, Takano K, Kanaya S, J Mol Biol. 2006 Feb 10;356(1):165-78. Epub 2005 Nov 28. PMID:16343535

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