2gra: Difference between revisions

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[[Image:2gra.gif|left|200px]]
{{Seed}}
[[Image:2gra.png|left|200px]]


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{{STRUCTURE_2gra|  PDB=2gra  |  SCENE=  }}  
{{STRUCTURE_2gra|  PDB=2gra  |  SCENE=  }}  


'''crystal structure of Human Pyrroline-5-carboxylate Reductase complexed with nadp'''
===crystal structure of Human Pyrroline-5-carboxylate Reductase complexed with nadp===




==Overview==
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Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.
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{{ABSTRACT_PUBMED_16730026}}


==About this Structure==
==About this Structure==
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[[Category: Human pyrroline-5-carboxylate reductase]]
[[Category: Human pyrroline-5-carboxylate reductase]]
[[Category: Nadph]]
[[Category: Nadph]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:25:44 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:14:33 2008''

Revision as of 12:14, 28 July 2008

File:2gra.png

Template:STRUCTURE 2gra

crystal structure of Human Pyrroline-5-carboxylate Reductase complexed with nadp

Template:ABSTRACT PUBMED 16730026

About this Structure

2GRA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:16730026

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