3b6c: Difference between revisions

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[[Image:3b6c.jpg|left|200px]]
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[[Image:3b6c.png|left|200px]]


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{{STRUCTURE_3b6c|  PDB=3b6c  |  SCENE=  }}  
{{STRUCTURE_3b6c|  PDB=3b6c  |  SCENE=  }}  


'''Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with (S)-DNPA'''
===Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with (S)-DNPA===




==Overview==
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Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is exported from the cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression. This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here, we report the structural basis for this precursor-mediated derepression with crystal structures of homodimeric ActR by itself and in complex with either actinorhodin or the actinorhodin biosynthetic intermediate (S)-DNPA [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti c acid]. The ligand-binding tunnel in each ActR monomer has a striking hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that accommodate either one hexacyclic actinorhodin molecule or two back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals the strongest structural evidence to date that TetR-mediated antibiotic resistance may have been acquired from an antibiotic-producer organism.
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{{ABSTRACT_PUBMED_18207163}}


==About this Structure==
==About this Structure==
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Transcriptional repressor]]
[[Category: Transcriptional repressor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:25:56 2008''
 
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Revision as of 12:23, 28 July 2008

File:3b6c.png

Template:STRUCTURE 3b6c

Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with (S)-DNPA

Template:ABSTRACT PUBMED 18207163

About this Structure

3B6C is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.

Reference

Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:18207163

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