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| [[Image:1phc.gif|left|200px]] | | {{Seed}} |
| | [[Image:1phc.png|left|200px]] |
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| {{STRUCTURE_1phc| PDB=1phc | SCENE= }} | | {{STRUCTURE_1phc| PDB=1phc | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450'''
| | ===CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450=== |
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| ==Overview==
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| The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one axial heme ligand and a water molecule or hydroxide ion providing the other axial ligand. A network of hydrogen-bonded solvent molecules occupies the substrate pocket in addition to the iron-linked aqua ligand. When a camphor molecule binds, the active site waters including the aqua ligand are displaced, resulting in a pentacoordinate high-spin heme iron atom. Analysis of the Fno camphor - F camphor difference Fourier and a quantitative comparison of the two refined structures reveal that no detectable conformational change results from camphor binding other than a small repositioning of a phenylalanine side chain that contacts the camphor molecule. However, large decreases in the mean temperature factors of three separate segments of the protein centered on Tyr-96, Thr-185, and Asp-251 result from camphor binding. This indicates that camphor binding decreases the flexibility in these three regions of the P-450cam molecule without altering the mean position of the atoms involved. | | The line below this paragraph, {{ABSTRACT_PUBMED_3768350}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 3768350 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_3768350}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Single protein]] | | [[Category: Single protein]] |
| [[Category: Poulos, T L.]] | | [[Category: Poulos, T L.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:05:23 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:35:51 2008'' |
