1t7v: Difference between revisions

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[[Image:1t7v.jpg|left|200px]]
{{Seed}}
[[Image:1t7v.png|left|200px]]


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{{STRUCTURE_1t7v|  PDB=1t7v  |  SCENE=  }}  
{{STRUCTURE_1t7v|  PDB=1t7v  |  SCENE=  }}  


'''Zn-alpha-2-glycoprotein; baculo-ZAG PEG 200'''
===Zn-alpha-2-glycoprotein; baculo-ZAG PEG 200===




==Overview==
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Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex (MHC) molecules and revealed a non-peptidic ligand in the ZAG counterpart of the MHC peptide-binding groove. Here we present crystallographic studies to explore further the nature of the non-peptidic ligand in the ZAG groove. Comparison of the structures of several forms of recombinant ZAG, including a 1.95 A structure derived from ZAG expressed in insect cells, suggests that the non-peptidic ligand in the current structures and in the structure of serum ZAG is a polyethylene glycol (PEG), which is present in the crystallization conditions used. Further support for PEG binding in the ZAG groove is provided by the finding that PEG displaces a fluorophore-tagged fatty acid from the ZAG binding site. From these results we hypothesize that our purified forms of ZAG do not contain a bound endogenous ligand, but that the ZAG groove is capable of binding hydrophobic molecules, which may relate to its function.
The line below this paragraph, {{ABSTRACT_PUBMED_15477100}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_15477100}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein., Delker SL, West AP Jr, McDermott L, Kennedy MW, Bjorkman PJ, J Struct Biol. 2004 Nov;148(2):205-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15477100 15477100]
Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein., Delker SL, West AP Jr, McDermott L, Kennedy MW, Bjorkman PJ, J Struct Biol. 2004 Nov;148(2):205-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15477100 15477100]
Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules., Sanchez LM, Chirino AJ, Bjorkman P, Science. 1999 Mar 19;283(5409):1914-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10206894 10206894]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Mhc class i homolog]]
[[Category: Mhc class i homolog]]
[[Category: Peg]]
[[Category: Peg]]
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