1sb0: Difference between revisions

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[[Image:1sb0.jpg|left|200px]]
{{Seed}}
[[Image:1sb0.png|left|200px]]


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{{STRUCTURE_1sb0|  PDB=1sb0  |  SCENE=  }}  
{{STRUCTURE_1sb0|  PDB=1sb0  |  SCENE=  }}  


'''Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb'''
===Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb===




==Overview==
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The hematopoietic transcription factor c-Myb activates transcription of target genes through direct interactions with the KIX domain of the co-activator CBP. The solution structure of the KIX domain in complex with the activation domain of c-Myb reveals a helical structure very similar to that adopted by KIX in complex with the phosphorylated kinase inducible domain (pKID) of CREB. While pKID contains two helices, alphaA and alphaB, which interact with KIX, the structure of bound c-Myb reveals a single bent amphipathic helix that binds in the same hydrophobic groove as the alphaB helix of pKID. The affinity of c-Myb for KIX is lower than that of pKID, and relies more heavily on optimal interactions of the single helix of c-Myb with residues in the hydrophobic groove. In particular, a deep hydrophobic pocket in KIX accounts for more than half the interactions with c-Myb observed by NMR. A bend in the alpha-helix of c-Myb enables a critical leucine side-chain to penetrate into this pocket more deeply than the equivalent leucine residue of pKID. The components that mediate the higher affinity of pKID for KIX, i.e. the phosphate group and the alphaA helix, are absent from c-Myb. Results from isothermal titration calorimetry, together with the structural data, point to a key difference between the two complexes in optimal pH for binding, as a result of differential pH-dependent interactions with histidine residues of KIX. These results explain the structural and thermodynamic basis for the observed constitutive versus inducible activation properties of c-Myb and CREB.
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{{ABSTRACT_PUBMED_15019774}}


==About this Structure==
==About this Structure==
1SB0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB0 OCA].  
1SB0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB0 OCA].  


==Reference==
==Reference==
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[[Category: Myb]]
[[Category: Myb]]
[[Category: Transcriptional activation]]
[[Category: Transcriptional activation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:29:52 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:10:03 2008''

Revision as of 13:10, 28 July 2008

File:1sb0.png

Template:STRUCTURE 1sb0

Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb

Template:ABSTRACT PUBMED 15019774

About this Structure

1SB0 is a Protein complex structure of sequences from Mus musculus. Full experimental information is available from OCA.

Reference

Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb., Zor T, De Guzman RN, Dyson HJ, Wright PE, J Mol Biol. 2004 Mar 26;337(3):521-34. PMID:15019774

Page seeded by OCA on Mon Jul 28 16:10:03 2008

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