2yt4: Difference between revisions

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[[Image:2yt4.~~gif~~|left|200px]]

[[Image:2yt4.png|left|200px]]

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~~'''~~Crystal structure of human DGCR8 core~~'''~~

===Crystal structure of human DGCR8 core===

~~==Overview==~~

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A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this ~~cleavage~~, ~~whereas DGCR8 or Pasha provides a frame for anchoring substrate pri-miRNAs. To clarify the molecular basis underlying recognition of pri-miRNA by DGCR8 and Pasha~~, ~~we determined~~ the ~~crystal structure of~~ the ~~human DGCR8 core~~ (~~DGCR8S, residues 493-720~~)~~. In the structure~~, the ~~two double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo two-fold symmetry and are tightly packed against the C-terminal helix~~. The H2 helix in each dsRBD is important for recognition of pri-miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri-~~miRNA in two possible orientations. We propose a model for DGCR8's recognition of pri~~-~~miRNA.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17704815}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17704815 is the PubMed ID number.

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==About this Structure==

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[[Category: Rna binding domain]]

[[Category: Rna binding protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 19~~:22:16 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:43:09 2008''

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-[[Image:2yt4.gif|left|200px]]
+{{Seed}}
+[[Image:2yt4.png|left|200px]]
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 {{STRUCTURE_2yt4|  PDB=2yt4  |  SCENE=  }}
-'''Crystal structure of human DGCR8 core'''
+===Crystal structure of human DGCR8 core===
-==Overview==
+<!--
-A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri-miRNAs. To clarify the molecular basis underlying recognition of pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo two-fold symmetry and are tightly packed against the C-terminal helix. The H2 helix in each dsRBD is important for recognition of pri-miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri-miRNA in two possible orientations. We propose a model for DGCR8's recognition of pri-miRNA.
+The line below this paragraph, {{ABSTRACT_PUBMED_17704815}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17704815 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17704815}}
 ==About this Structure==
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 [[Category: Rna binding domain]]
 [[Category: Rna binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 19:22:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:43:09 2008''