1x9e: Difference between revisions

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[[Image:1x9e.jpg|left|200px]]
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{{STRUCTURE_1x9e|  PDB=1x9e  |  SCENE=  }}  
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'''Crystal structure of HMG-CoA synthase from Enterococcus faecalis'''
===Crystal structure of HMG-CoA synthase from Enterococcus faecalis===




==Overview==
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Biosynthesis of the isoprenoid precursor, isopentenyl diphosphate, is a critical function in all independently living organisms. There are two major pathways for this synthesis, the non-mevalonate pathway found in most eubacteria and the mevalonate pathway found in animal cells and a number of pathogenic bacteria. An early step in this pathway is the condensation of acetyl-CoA and acetoacetyl-CoA into HMG-CoA, catalyzed by the enzyme HMG-CoA synthase. To explore the possibility of a small molecule inhibitor of the enzyme functioning as a non-cell wall antibiotic, the structure of HMG-CoA synthase from Enterococcus faecalis (MVAS) was determined by selenomethionine MAD phasing to 2.4 A and the enzyme complexed with its second substrate, acetoacetyl-CoA, to 1.9 A. These structures show that HMG-CoA synthase from Enterococcus is a member of the family of thiolase fold enzymes and, while similar to the recently published HMG-CoA synthase structures from Staphylococcus aureus, exhibit significant differences in the structure of the C-terminal domain. The acetoacetyl-CoA binary structure demonstrates reduced coenzyme A and acetoacetate covalently bound to the active site cysteine through a thioester bond. This is consistent with the kinetics of the reaction that have shown acetoacetyl-CoA to be a potent inhibitor of the overall reaction, and provides a starting point in the search for a small molecule inhibitor.
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{{ABSTRACT_PUBMED_16245942}}


==About this Structure==
==About this Structure==
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[[Category: Vartia, A A.]]
[[Category: Vartia, A A.]]
[[Category: Thiolase family]]
[[Category: Thiolase family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:08:39 2008''

Revision as of 14:08, 28 July 2008

File:1x9e.png

Template:STRUCTURE 1x9e

Crystal structure of HMG-CoA synthase from Enterococcus faecalis

Template:ABSTRACT PUBMED 16245942

About this Structure

1X9E is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of HMG-CoA synthase from Enterococcus faecalis and a complex with its second substrate/inhibitor acetoacetyl-CoA., Steussy CN, Vartia AA, Burgner JW 2nd, Sutherlin A, Rodwell VW, Stauffacher CV, Biochemistry. 2005 Nov 1;44(43):14256-67. PMID:16245942

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