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[[Image:2pak.gif|left|200px]]
{{Seed}}
[[Image:2pak.png|left|200px]]


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{{STRUCTURE_2pak|  PDB=2pak  |  SCENE=  }}  
{{STRUCTURE_2pak|  PDB=2pak  |  SCENE=  }}  


'''Structure of a H51N mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP'''
===Structure of a H51N mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP===




==Overview==
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The repeating unit of the glycan chain in the S-layer of the bacterium Aneurinibacillus thermoaerophilus L420-91(T) is composed of four alpha-d-rhamnose molecules and two 3-acetamido-3,6-dideoxy-alpha-d-galactose moieties (abbreviated as Fucp3NAc). Formation of the glycan layer requires nucleotide-activated sugars as the donor molecules. Whereas the enzymes involved in the synthesis of GDP-rhamnose have been well characterized, less is known regarding the structures and enzymatic mechanisms of the enzymes required for the production of dTDP-Fucp3NAc. One of the enzymes involved in the biosynthesis of dTDP-Fucp3NAc is a 3,4-ketoisomerase, hereafter referred to as FdtA. Here we describe the first three-dimensional structure of this sugar isomerase complexed with dTDP and solved to 1.5 A resolution. The FdtA dimer assumes an almost jellyfish-like appearance with the sole alpha-helices representing the tentacles. Formation of the FdtA dimer represents a classical example of domain swapping whereby beta-strands 2 and 3 from one subunit form part of a beta-sheet in the second subunit. The active site architecture of FdtA is characterized by a cluster of three histidine residues, two of which, His(49) and His(51), appear to be strictly conserved in the amino acid sequences deposited to date. Site-directed mutagenesis experiments, enzymatic assays, and x-ray crystallographic analyses suggest that His(49) functions as an active site base.
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{{ABSTRACT_PUBMED_17459872}}


==About this Structure==
==About this Structure==
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[[Category: Ketoisomerase]]
[[Category: Ketoisomerase]]
[[Category: S-layer biosynthesis]]
[[Category: S-layer biosynthesis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 12:43:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:42:06 2008''

Revision as of 15:42, 28 July 2008

File:2pak.png

Template:STRUCTURE 2pak

Structure of a H51N mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP

Template:ABSTRACT PUBMED 17459872

About this Structure

2PAK is a Single protein structure of sequence from Aneurinibacillus thermoaerophilus. Full crystallographic information is available from OCA.

Reference

The x-ray structure of dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase., Davis ML, Thoden JB, Holden HM, J Biol Chem. 2007 Jun 29;282(26):19227-36. Epub 2007 Apr 25. PMID:17459872

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