3b9d: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:3b9d.jpg|left|200px]]
{{Seed}}
[[Image:3b9d.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3b9d|  PDB=3b9d  |  SCENE=  }}  
{{STRUCTURE_3b9d|  PDB=3b9d  |  SCENE=  }}  


'''Crystal structure of Vibrio harveyi chitinase A complexed with pentasaccharide'''
===Crystal structure of Vibrio harveyi chitinase A complexed with pentasaccharide===




==Overview==
<!--  
This research describes four X-ray structures of Vibrio harveyi chitinase A and its catalytically inactive mutant (E315M) in the presence and absence of substrates. The overall structure of chitinase A is that of a typical family-18 glycosyl hydrolase comprising three distinct domains: (i) the amino-terminal chitin-binding domain; (ii) the main catalytic (alpha/beta)(8) TIM-barrel domain; and (iii) the small (alpha+beta) insertion domain. The catalytic cleft of chitinase A has a long, deep groove, which contains six chitooligosaccharide ring-binding subsites (-4)(-3)(-2)(-1)(+1)(+2). The binding cleft of the ligand-free E315M is partially blocked by the C-terminal (His)(6)-tag. Structures of E315M-chitooligosaccharide complexes display a linear conformation of pentaNAG, but a bent conformation of hexaNAG. Analysis of the final 2F(o)-F(c) omit map of E315M-NAG6 reveals the existence of the linear conformation of the hexaNAG at a lower occupancy with respect to the bent conformation. These crystallographic data provide evidence that the interacting sugars undergo conformational changes prior to hydrolysis by the wild-type enzyme.
The line below this paragraph, {{ABSTRACT_PUBMED_18467126}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 18467126 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_18467126}}


==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Pentasaccharide complex]]
[[Category: Pentasaccharide complex]]
[[Category: Tim-barrel]]
[[Category: Tim-barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:20:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:32:30 2008''

Revision as of 16:32, 28 July 2008

File:3b9d.png

Template:STRUCTURE 3b9d

Crystal structure of Vibrio harveyi chitinase A complexed with pentasaccharide

Template:ABSTRACT PUBMED 18467126

About this Structure

3B9D is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.

Reference

Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism., Songsiriritthigul C, Pantoom S, Aguda AH, Robinson RC, Suginta W, J Struct Biol. 2008 Jun;162(3):491-9. Epub 2008 Mar 26. PMID:18467126

Page seeded by OCA on Mon Jul 28 19:32:30 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3b9d&oldid=712252"