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| {{STRUCTURE_1tfe| PDB=1tfe | SCENE= }} | | {{STRUCTURE_1tfe| PDB=1tfe | SCENE= }} |
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| '''DIMERIZATION DOMAIN OF EF-TS FROM T. THERMOPHILUS'''
| | ===DIMERIZATION DOMAIN OF EF-TS FROM T. THERMOPHILUS=== |
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| ==Overview==
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| Elongation factor Ts (EF-Ts) functions as a nucleotide-exchange factor by binding elongation factor Tu (EF-Tu) and accelerating the GDP dissociation from EF-Tu; thus EF-Ts promotes the transition of EF-Tu from the inactive GDP form to the active GTP form. Thermus thermophilus EF-Ts exists as a stable dimer in solution which binds two molecules of EF-Tu to form a (EF-Tu.EF-Ts)2 heterotetramer. Here we report the crystal structure of the dimerization domain of EF-Ts from T. thermophilus refined to 1.7 A resolution. A three-stranded antiparallel beta-sheet from each subunit interacts to form a beta-sandwich that serves as an extensive dimer interface tethered by a disulfide bond. This interface is distinctly different from the predominantly alpha-helical one that stabilizes the EF-Ts dimer from Escherichia coli [Kawashima, T., et al. (1996) Nature 379, 511-518]. To test whether the homodimeric form of T. thermophilus EF-Ts is necessary for catalyzing nucleotide exchange, the present structure was used to design mutational changes within the dimer interface that disrupt the T. thermophilus EF-Ts dimer but not the tertiary structure of the subunits. Surprisingly, EF-Ts monomers created in this manner failed to catalyze nucleotide exchange in EF-Tu, indicating that, in vitro. T. thermophilus EF-Ts functions only as a homodimer.
| | The line below this paragraph, {{ABSTRACT_PUBMED_8756682}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8756682 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8756682}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sprinzl, M.]] | | [[Category: Sprinzl, M.]] |
| [[Category: Elongation factor]] | | [[Category: Elongation factor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:53:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:51:30 2008'' |
