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[[Image:1nso.gif|left|200px]]
{{Seed}}
[[Image:1nso.png|left|200px]]


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{{STRUCTURE_1nso|  PDB=1nso  |  SCENE=  }}  
{{STRUCTURE_1nso|  PDB=1nso  |  SCENE=  }}  


'''Folded monomer of protease from Mason-Pfizer monkey virus'''
===Folded monomer of protease from Mason-Pfizer monkey virus===




==Overview==
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The assembly of Mason-Pfizer monkey virus Gag polyproteins into immature capsids and their cleavage by the encoded protease are temporally and spatially separated processes, making the virus a particularly useful model for investigation of protease activation. Here we present a high resolution NMR structure of a fully folded monomer of a 12 kDa M-PMV protease (wt 12 PR) and of a Cys7Ala/Asp26Asn/Cys106Ala mutant (12 PR(D26N/C7A/C106A)). The overall structures of both wt 12 PR and 12 PR(D26N/C7A/C106A) follow the conservative structural motif of other retroviral proteases. The most prominent difference from the canonical fold of retroviral proteases is the absence of the interfacial beta-sheet, which leads to the loss of the principal force stabilizing the dimer of M-PMV PR. The monomer-dimer equilibrium can be shifted in favor of the dimer by adding a substrate or an inhibitor, partially compensating for the missing role of the beta-sheet. We also show that cysteines C7 and C106 play a crucial role in stabilizing the dimer and consequently increasing the proteolytic activity of M-PMV PR. This is consistent with the role of reversible oxidative modification of the cysteine residues in the regulation of the maturation of assembled M-PMV capsids in the cytoplasm.
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{{ABSTRACT_PUBMED_14568536}}


==About this Structure==
==About this Structure==
1NSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_retrovirus_1 Simian retrovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSO OCA].  
1NSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_retrovirus_1 Simian retrovirus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSO OCA].  


==Reference==
==Reference==
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[[Category: Protease]]
[[Category: Protease]]
[[Category: Virus maturation]]
[[Category: Virus maturation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:55:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:16:09 2008''

Revision as of 18:16, 28 July 2008

File:1nso.png

Template:STRUCTURE 1nso

Folded monomer of protease from Mason-Pfizer monkey virus

Template:ABSTRACT PUBMED 14568536

About this Structure

1NSO is a Single protein structure of sequence from Simian retrovirus 1. Full experimental information is available from OCA.

Reference

Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:14568536

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