2o4f: Difference between revisions

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[[Image:2o4f.gif|left|200px]]
{{Seed}}
[[Image:2o4f.png|left|200px]]


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{{STRUCTURE_2o4f|  PDB=2o4f  |  SCENE=  }}  
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'''Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions'''
===Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions===




==Overview==
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A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement. The asymmetric unit consists of a characteristic motif of two quadruplexes stacked at their 5' ends. All nucleotides are clearly defined in the density and could be positioned. A single bound Li(+) ion is observed at the surface of the column formed by the two joined molecules. Thus, this small alkali metal ion appears to be unsuitable as a replacement for the Na(+) ion in the central channel of G-quartets, unlike K(+) or Tl(+) ions. A well conserved constellation of water molecules is observed in the grooves of the dimeric structure.
The line below this paragraph, {{ABSTRACT_PUBMED_17505106}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_17505106}}


==About this Structure==
==About this Structure==
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[[Category: Parallel-stranded]]
[[Category: Parallel-stranded]]
[[Category: Quadruple helix]]
[[Category: Quadruple helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:16:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:27:42 2008''

Revision as of 18:27, 28 July 2008

File:2o4f.png

Template:STRUCTURE 2o4f

Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions

Template:ABSTRACT PUBMED 17505106

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions., Creze C, Rinaldi B, Haser R, Bouvet P, Gouet P, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):682-8. Epub 2007, May 15. PMID:17505106

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