1o7k: Difference between revisions

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[[Image:1o7k.gif|left|200px]]
{{Seed}}
[[Image:1o7k.png|left|200px]]


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{{STRUCTURE_1o7k|  PDB=1o7k  |  SCENE=  }}  
{{STRUCTURE_1o7k|  PDB=1o7k  |  SCENE=  }}  


'''HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES'''
===HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES===




==Overview==
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p47(phox) is a key cytosolic subunit required for activation of phagocyte NADPH oxidase. The X-ray structure of the p47(phox) PX domain revealed two distinct basic pockets on the membrane-binding surface, each occupied by a sulfate. These two pockets have different specificities: one preferentially binds phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P(2)] and is analogous to the phophatidylinositol 3-phosphate (PtdIns3P)-binding pocket of p40(phox), while the other binds anionic phospholipids such as phosphatidic acid (PtdOH) or phosphatidylserine. The preference of this second site for PtdOH may be related to previously observed activation of NADPH oxidase by PtdOH. Simultaneous occupancy of the two phospholipid-binding pockets radically increases membrane affinity. Strikingly, measurements for full-length p47(phox) show that membrane interaction by the PX domain is masked by an intramolecular association with the C-terminal SH3 domain (C-SH3). Either a site-specific mutation in C-SH3 (W263R) or a mimic of the phosphorylated form of p47(phox) [Ser(303, 304, 328, 359, 370)Glu] cause a transition from a closed to an open conformation that binds membranes with a greater affinity than the isolated PX domain.
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{{ABSTRACT_PUBMED_12356722}}


==About this Structure==
==About this Structure==
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[[Category: Px domain]]
[[Category: Px domain]]
[[Category: Sh3 domain]]
[[Category: Sh3 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:46:36 2008''

Revision as of 18:46, 28 July 2008

File:1o7k.png

Template:STRUCTURE 1o7k

HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES

Template:ABSTRACT PUBMED 12356722

About this Structure

1O7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction., Karathanassis D, Stahelin RV, Bravo J, Perisic O, Pacold CM, Cho W, Williams RL, EMBO J. 2002 Oct 1;21(19):5057-68. PMID:12356722

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