2j7u: Difference between revisions

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[[Image:2j7u.gif|left|200px]]
{{Seed}}
[[Image:2j7u.png|left|200px]]


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{{STRUCTURE_2j7u|  PDB=2j7u  |  SCENE=  }}  
{{STRUCTURE_2j7u|  PDB=2j7u  |  SCENE=  }}  


'''DENGUE VIRUS NS5 RNA DEPENDENT RNA POLYMERASE DOMAIN'''
===DENGUE VIRUS NS5 RNA DEPENDENT RNA POLYMERASE DOMAIN===




==Overview==
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Dengue fever, a neglected emerging disease for which no vaccine or antiviral agents exist at present, is caused by dengue virus, a member of the Flavivirus genus, which includes several important human pathogens, such as yellow fever and West Nile viruses. The NS5 protein from dengue virus is bifunctional and contains 900 amino acids. The S-adenosyl methionine transferase activity resides within its N-terminal domain, and residues 270 to 900 form the RNA-dependent RNA polymerase (RdRp) catalytic domain. Viral replication begins with the synthesis of minus-strand RNA from the dengue virus positive-strand RNA genome, which is subsequently used as a template for synthesizing additional plus-strand RNA genomes. This essential function for the production of new viral particles is catalyzed by the NS5 RdRp. Here we present a high-throughput in vitro assay partly recapitulating this activity and the crystallographic structure of an enzymatically active fragment of the dengue virus RdRp refined at 1.85-A resolution. The NS5 nuclear localization sequences, previously thought to fold into a separate domain, form an integral part of the polymerase subdomains. The structure also reveals the presence of two zinc ion binding motifs. In the absence of a template strand, a chain-terminating nucleoside analogue binds to the priming loop site. These results should inform and accelerate the structure-based design of antiviral compounds against dengue virus.
The line below this paragraph, {{ABSTRACT_PUBMED_17301146}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17301146 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17301146}}


==About this Structure==
==About this Structure==
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[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Viral protein]]
[[Category: Viral protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:06:57 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:55:08 2008''

Revision as of 18:55, 28 July 2008

File:2j7u.png

Template:STRUCTURE 2j7u

DENGUE VIRUS NS5 RNA DEPENDENT RNA POLYMERASE DOMAIN

Template:ABSTRACT PUBMED 17301146

About this Structure

2J7U is a Single protein structure of sequence from Dengue virus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution., Yap TL, Xu T, Chen YL, Malet H, Egloff MP, Canard B, Vasudevan SG, Lescar J, J Virol. 2007 May;81(9):4753-65. Epub 2007 Feb 14. PMID:17301146

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