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| [[Image:1v7l.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1v7l| PDB=1v7l | SCENE= }} | | {{STRUCTURE_1v7l| PDB=1v7l | SCENE= }} |
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| '''Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii'''
| | ===Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii=== |
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| ==Overview==
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| Recent studies have implied that the isopropylmalate isomerase small subunit of the hyperthermophilic archaea Pyrococcus horikoshii (PhIPMI-s) functions as isopropylmalate isomerase in the leucine biosynthesis pathway, and as homoaconitase (HACN) in the lysine biosynthesis pathway via alpha-aminoadipic acid. PhIPMI is thus considered a key to understanding the fundamental metabolism of the earliest organisms. We describe for the first time the crystal structure of PhIPMI-s, which displays dual substrate specificity. The crystal structure unexpectedly shows that four molecules create an interlocked assembly with intermolecular disulfide linkages having a skewed 222 point-group symmetry. Although the overall fold of the PhIPMI-s monomer is related closely to domain 4 of the aconitase (ACN), one alpha-helix in the ACN structure is replaced by a short loop with relatively high temperature factor values. Because this region is essential for discriminating the structurally similar substrate based on interactions with its diversified gamma-moiety, the loop structure in the PhIPMI-s must be dependent on the presence of a substrate. The flexibility of the loop region might be a structural basis for recognizing both hydrophobic and hydrophilic gamma-moieties of two distinct substrates, isopropylmalate and homocitrate.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15522288}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15522288 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15522288}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Yao, M.]] | | [[Category: Yao, M.]] |
| [[Category: Beta barrel]] | | [[Category: Beta barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:10:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:20:27 2008'' |
