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| [[Image:2itl.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2itl| PDB=2itl | SCENE= }} | | {{STRUCTURE_2itl| PDB=2itl | SCENE= }} |
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| '''The origin binding domain of the SV40 large T antigen bound to the functional pen palindrome DNA (23 bp)'''
| | ===The origin binding domain of the SV40 large T antigen bound to the functional pen palindrome DNA (23 bp)=== |
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| ==Overview==
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| The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown to interact with the DNA through a loop comprising Ser147-Thr155 (A1 loop), a combination of a DNA-binding helix and loop (His203-Asn210), and Asn227. The A1 loop traveled back-and-forth along the major groove and accounted for most of the sequence-determining contacts with the DNA. Unexpectedly, in both T-ag-DNA structures, the T-ag OBDs bound DNA independently and did not make direct protein-protein contacts. The T-ag OBD was also captured bound to a non-consensus site ATGGC even in the presence of its canonical site GAGGC. Our observations taken together with the known biochemical and structural features of the T-ag-origin interaction suggest a model for origin unwinding. | | The line below this paragraph, {{ABSTRACT_PUBMED_17139255}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17139255 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17139255}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Seitova, A]] | | [[Category: Seitova, A]] |
| [[Category: Dna binding protein]] | | [[Category: Dna binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:51:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:26:49 2008'' |
