1tks: Difference between revisions

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[[Image:1tks.gif|left|200px]]
{{Seed}}
[[Image:1tks.png|left|200px]]


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{{STRUCTURE_1tks|  PDB=1tks  |  SCENE=  }}  
{{STRUCTURE_1tks|  PDB=1tks  |  SCENE=  }}  


'''Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans'''
===Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans===




==Overview==
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A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
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{{ABSTRACT_PUBMED_15328619}}


==About this Structure==
==About this Structure==
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[[Category: Crystal structure synthetic gene]]
[[Category: Crystal structure synthetic gene]]
[[Category: Riboflavin biosynthesis]]
[[Category: Riboflavin biosynthesis]]
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