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| {{STRUCTURE_1t2t| PDB=1t2t | SCENE= }} | | {{STRUCTURE_1t2t| PDB=1t2t | SCENE= }} |
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| '''Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site'''
| | ===Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site=== |
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| ==Overview==
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| Customary binding sites of intron-encoded homing endonucleases lie within cognate intronless alleles, at the so-called homing sites. Here, we describe a novel, high-affinity binding site for I-TevI endonuclease, encoded within the group I td intron of phage T4. This site is an operator that overlaps the T4 late promoter, which drives I-TevI expression from within the td intron. I-TevI binds the operator and homing sites with equal affinity, and functions as a transcriptional autorepressor. Distinct sequence and spacing requirements of the catalytic domain result in reduced cleavage activity on operator DNA. Crystallographic studies showed that the overall interactions of the DNA-binding domain with the operator and homing sites are similar, but have some different hydrogen-bonding contacts. We present a model in which the flexibility in protein-DNA interactions allows I-TevI to bind variant intronless alleles to promote intron mobility while facilitating its function in autorepression, and thereby persistence in its host.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15361856}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15361856 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15361856}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Stanger, M J.]] | | [[Category: Stanger, M J.]] |
| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:26:48 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:50:41 2008'' |
