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| {{STRUCTURE_1zof| PDB=1zof | SCENE= }} | | {{STRUCTURE_1zof| PDB=1zof | SCENE= }} |
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| '''Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori'''
| | ===Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori=== |
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| ==Overview==
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| The AhpC protein from H. pylori, a thioredoxin (Trx)-dependent alkyl hydroperoxide-reductase, is a member of the ubiquitous 2-Cys peroxiredoxins family (2-Cys Prxs), a group of thiol-specific antioxidant enzymes. Prxs exert the protective antioxidant role in cells through their peroxidase activity, whereby hydrogen peroxide, peroxynitrite and a wide range of organic hydroperoxides (ROOH) are reduced and detoxified (ROOH + 2e(-)-->ROH + H2O). In this study AhpC has been cloned and overexpressed in E. coli. After purification to homogeneity, crystals of the recombinant protein were grown. They diffract to 2.95 A resolution using synchrotron radiation. The crystal structure of AhpC has been determined using the molecular replacement method (R = 23.6%, R(free) = 25.9%). The model, similar in the overall to other members of the 2-Cys Prx family crystallized as toroide-shaped complexes, consists of a pentameric arrangement of homodimers [(alpha2)5 decamer]. The model of AhpC from H. pylori presents significant differences with respect to other members of the family: apart from some loop regions, alpha5-helix and the C-terminus is shifted, preventing the C-terminal tail of the second subunit from extending toward this region of the molecule. Oligomerization properties of AhpC have been also characterized by gel filtration chromatography. | | The line below this paragraph, {{ABSTRACT_PUBMED_16213196}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16213196 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16213196}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Decamer]] | | [[Category: Decamer]] |
| [[Category: Toroide-shaped complex]] | | [[Category: Toroide-shaped complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:31:07 2008'' |
