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| [[Image:2hh7.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2hh7| PDB=2hh7 | SCENE= }} | | {{STRUCTURE_2hh7| PDB=2hh7 | SCENE= }} |
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| '''Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.'''
| | ===Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.=== |
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| ==Overview==
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| Copper is an essential element that becomes highly cytotoxic when concentrations exceed the capacity of cells to sequester the ion. Here, we identify a new copper-specific repressor (CsoR) of a copper-sensitive operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of a large, previously uncharacterized family of proteins (DUF156). Electronic and X-ray absorption spectroscopies reveal that CsoR binds a single-monomer mole equivalent of Cu(I) to form a trigonally coordinated (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR shows a homodimeric antiparallel four-helix bundle architecture that represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively regulates the binding of CsoR to a DNA fragment encompassing the operator-promoter region of the Mtb cso operon; this results in derepression of the operon in Mtb and the heterologous host Mycobacterium smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17143269}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17143269 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17143269}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sacchettini,J C.]] | | [[Category: Sacchettini,J C.]] |
| [[Category: 4-helix bundle]] | | [[Category: 4-helix bundle]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:17:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:40:41 2008'' |
