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| [[Image:2vq0.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2vq0| PDB=2vq0 | SCENE= }} | | {{STRUCTURE_2vq0| PDB=2vq0 | SCENE= }} |
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| '''CAPSID STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN DELETION MUTANT RCP(DELTA 48 TO 59)'''
| | ===CAPSID STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN DELETION MUTANT RCP(DELTA 48 TO 59)=== |
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| ==Overview==
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| A unique feature of several T=3 icosahedral viruses is the presence of a structure called the beta-annulus formed by extensive hydrogen bonding between protein subunits related by icosahedral three-fold axis of symmetry. This unique structure has been suggested as a molecular switch that determines the T=3 capsid assembly. In order to examine the importance of the beta-annulus, a deletion mutant of Sesbania mosaic virus coat protein in which residues 48-59 involved in the formation of the beta-annulus were deleted retaining the rest of the residues in the amino terminal segment (rCP (Delta48-59)) was constructed. When expressed in Escherichia coli, the mutant protein assembled into virus like particles of sizes close to that of the wild type virus particles. The purified capsids were crystallized and their three dimensional structure was determined at 3.6 A resolution by X-ray crystallography. The mutant capsid structure closely resembled that of the native virus particles. However, surprisingly, the structure revealed that the assembly of the particles has proceeded without the formation of the beta-annulus. Therefore, the beta-annulus is not essential for T=3 capsid assembly as speculated earlier and may be formed as a consequence of the particle assembly. This is the first structural demonstration that the virus particle morphology with and without the beta-annulus could be closely similar.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18295296}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18295296 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18295296}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Virion]] | | [[Category: Virion]] |
| [[Category: Virus assembly]] | | [[Category: Virus assembly]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:27:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:54:08 2008'' |
