2r5c: Difference between revisions

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[[Image:2r5c.jpg|left|200px]]
{{Seed}}
[[Image:2r5c.png|left|200px]]


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{{STRUCTURE_2r5c|  PDB=2r5c  |  SCENE=  }}  
{{STRUCTURE_2r5c|  PDB=2r5c  |  SCENE=  }}  


'''Aedes Kynurenine Aminotransferase in Complex with Cysteine'''
===Aedes Kynurenine Aminotransferase in Complex with Cysteine===




==Overview==
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Aedes aegypti kynurenine aminotransferase (AeKAT) is a multifunctional aminotransferase. It catalyzes the transamination of a number of amino acids and uses many biologically relevant alpha-keto acids as amino group acceptors. AeKAT also is a cysteine S-conjugate beta-lyase. The most important function of AeKAT is the biosynthesis of kynurenic acid, a natural antagonist of NMDA and alpha7-nicotinic acetylcholine receptors. Here, we report the crystal structures of AeKAT in complex with its best amino acid substrates, glutamine and cysteine. Glutamine is found in both subunits of the biological dimer, and cysteine is found in one of the two subunits. Both substrates form external aldemines with pyridoxal 5-phosphate in the structures. This is the first instance in which one pyridoxal 5-phosphate enzyme has been crystallized with cysteine or glutamine forming external aldimine complexes, cysteinyl aldimine and glutaminyl aldimine. All the units with substrate are in the closed conformation form, and the unit without substrate is in the open form, which suggests that the binding of substrate induces the conformation change of AeKAT. By comparing the active site residues of the AeKAT-cysteine structure with those of the human KAT I-phenylalanine structure, we determined that Tyr286 in AeKAT is changed to Phe278 in human KAT I, which may explain why AeKAT transaminates hydrophilic amino acids more efficiently than human KAT I does.
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{{ABSTRACT_PUBMED_18186649}}


==About this Structure==
==About this Structure==
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[[Category: Aminotransferase]]
[[Category: Aminotransferase]]
[[Category: Pyridoxal-5-phosphate dependent transferase]]
[[Category: Pyridoxal-5-phosphate dependent transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:16:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:46:04 2008''

Revision as of 22:46, 28 July 2008

File:2r5c.png

Template:STRUCTURE 2r5c

Aedes Kynurenine Aminotransferase in Complex with Cysteine

Template:ABSTRACT PUBMED 18186649

About this Structure

2R5C is a Protein complex structure of sequences from Aedes aegypti. Full crystallographic information is available from OCA.

Reference

Structural insight into the mechanism of substrate specificity of aedes kynurenine aminotransferase., Han Q, Gao YG, Robinson H, Li J, Biochemistry. 2008 Feb 12;47(6):1622-30. Epub 2008 Jan 11. PMID:18186649

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