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| {{STRUCTURE_1uad| PDB=1uad | SCENE= }} | | {{STRUCTURE_1uad| PDB=1uad | SCENE= }} |
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| '''Crystal structure of the RalA-GppNHp-Sec5 Ral-binding domain complex'''
| | ===Crystal structure of the RalA-GppNHp-Sec5 Ral-binding domain complex=== |
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| ==Overview==
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| The sec6/8 complex or exocyst is an octameric protein complex that functions during cell polarization by regulating the site of exocytic vesicle docking to the plasma membrane, in concert with small GTP-binding proteins. The Sec5 subunit of the mammalian sec6/8 complex binds Ral in a GTP-dependent manner. Here we report the crystal structure of the complex between the Ral-binding domain of Sec5 and RalA bound to a non-hydrolyzable GTP analog (GppNHp) at 2.1 A resolution, providing the first structural insights into the mechanism and specificity of sec6/8 regulation. The Sec5 Ral-binding domain folds into an immunoglobulin-like beta-sandwich structure, which represents a novel fold for an effector of a GTP-binding protein. The interface between the two proteins involves a continuous antiparallel beta-sheet, similar to that found in other effector/G-protein complexes, such as Ras and Rap1A. Specific interactions unique to the RalA.Sec5 complex include Sec5 Thr11 and Arg27, and RalA Glu38, which we show are required for complex formation by isothermal titration calorimetry. Comparison of the structures of GppNHp- and GDP-bound RalA suggests a nucleotide-dependent switch mechanism for Sec5 binding. | | The line below this paragraph, {{ABSTRACT_PUBMED_12839989}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12839989 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12839989}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Immunogloblin-like fold]] | | [[Category: Immunogloblin-like fold]] |
| [[Category: Small gtp-binding protein]] | | [[Category: Small gtp-binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:57:53 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:14:38 2008'' |
