2a8g: Difference between revisions

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[[Image:2a8g.gif|left|200px]]
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[[Image:2a8g.png|left|200px]]


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{{STRUCTURE_2a8g|  PDB=2a8g  |  SCENE=  }}  
{{STRUCTURE_2a8g|  PDB=2a8g  |  SCENE=  }}  


'''Structure of Avidin in complex with the ligand deoxyguanosine'''
===Structure of Avidin in complex with the ligand deoxyguanosine===




==Overview==
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Oxidative damage of DNA results in the formation of many products, including 8-oxodeoxyguanosine, which has been used as a marker to quantify DNA damage. Earlier studies have demonstrated that avidin, a protein prevalent in egg-white and which has high affinity for the vitamin biotin, binds to 8-oxodeoxyguanosine and related bases. In this study, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeoxyadenosine. In each case, the base is observed to bind within the biotin-binding site of avidin. However, the mode of association between the bases and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein in this region does not accompany binding. Fluorescence studies indicate that in solution the individual bases, and a range of oligonucleotides, bind to avidin with micromolar affinity. Only one of the modes of binding observed is consistent with recognition of oxidised purines when incorporated within a DNA oligomer, and from this structure a model is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanosine. These studies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the low levels of binding observed are inconsistent with the recognition of oxidised purines forming a major physiological role for avidin.
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{{ABSTRACT_PUBMED_16413579}}


==About this Structure==
==About this Structure==
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[[Category: Thomas, S.]]
[[Category: Thomas, S.]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:44:39 2008''
 
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Revision as of 23:26, 28 July 2008

File:2a8g.png

Template:STRUCTURE 2a8g

Structure of Avidin in complex with the ligand deoxyguanosine

Template:ABSTRACT PUBMED 16413579

About this Structure

2A8G is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Recognition of oxidatively modified bases within the biotin-binding site of avidin., Conners R, Hooley E, Clarke AR, Thomas S, Brady RL, J Mol Biol. 2006 Mar 17;357(1):263-74. Epub 2006 Jan 6. PMID:16413579

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