|
|
| Line 1: |
Line 1: |
| [[Image:2bf8.gif|left|200px]] | | {{Seed}} |
| | [[Image:2bf8.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2bf8| PDB=2bf8 | SCENE= }} | | {{STRUCTURE_2bf8| PDB=2bf8 | SCENE= }} |
|
| |
|
| '''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''
| | ===CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15723079}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15723079 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15723079}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 42: |
Line 46: |
| [[Category: Sumo]] | | [[Category: Sumo]] |
| [[Category: Sumo-target structure]] | | [[Category: Sumo-target structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:54 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:39:41 2008'' |
